allendalepharm Chapter 308 Artificial Organelles!
Chapter 308 Artificial Organelles!
Yu Anbang helped raise algae for an hour and left in a hurry.
Song He continued to work alone, replenishing the nutrients in each culture bottle and observing repeatedly with a microscope.
At three o'clock in the afternoon, dozens of culture bottles had turned green, and the turbid green was floating in the bottles like trapped mist.
Finally, the black technology blueprint pops up!
The progress bar advances to 10%. There are enough algae in the laboratory. Most of the algae in the culture bottles weigh close to one kilogram. The first step of "looking for algae" is completed!
Song He checks the next step of the blueprint, which takes up a full 30% of the progress bar.
[Artificial organelles: Appropriate artificial organelles are implanted into algae to give them the characteristics of nitrifying bacteria, which can oxidize ammonia into nitrite and nitrite into nitric acid, thereby enhancing their own energy intake]
"What a risk!" Song He broke into a cold sweat.
He turned to look at the corner of the laboratory. A huge instrument like a piano was lying quietly. It was an all-in-one organelle machine.
This machine is extremely expensive, with a price of a terrifying 40 million yuan. Even after using it as a discounted second-hand product, it is still worth 28 million yuan. In addition, the material cost for each startup is at least 10,000 yuan, so he reserved 3 million yuan of materials to severely limit his scientific research. A lot of money is spent!
He had considered not wanting this machine. After all, artificial organelles were very niche and would become luxury items if they were no longer used. But after hesitating, he still gritted his teeth and asked for it.
It’s too risky! Fortunately, I asked for it, otherwise I would not be able to pass this level!
Song He started work, picked out a batch of diatom samples, separated out about 500 grams, and poured it into the organelle all-in-one machine.
Then he opened the ultra-low temperature refrigerator, found a few bottles of reagents in the white air, and poured them into the machine with trembling hands.
Why are my hands shaking? Is it cold?
Of course not, it’s fear!
These small bottles of reagents are more expensive than gold, and one drop can cost four digits. If your hand loosens and falls to pieces on the floor, just the sight of it will cause pain in your brain!
There was no danger, and all the reagents were poured into the machine, not a drop left.
Song He Press the screen of the all-in-one machine to turn it on!
Amidst the buzzing sound, the instrument performs self-tests and separates the algae liquid and reagents that have just been poured in. The faint sound of rushing water can be heard, and the movement looks like a person hiding in the machine to urinate.
Taking advantage of the self-test of the instrument, Song He recalled relevant knowledge about artificial organelles.
The scientist who realized the artificial synthesis of organelles for the first time was Dr. Guo Haotian of Xunzhu Technology Company. The Gaul Pasteur Center for Superstructural Bioimaging and the Shenzhen Institute of Synthetic Biology also made great contributions, ushering in the era of artificial organelles for humans.
Current technology is still limited to the field of membraneless organelles, that is, biological macromolecules aggregate into droplets, resulting in liquid phase separation. The entire process is very similar to oil-water separation.
The all-in-one organelle machine is the product of this technology. This machine can accurately synthesize artificial organelles and try to inject them into target cells.
Right now, there are two difficulties for Song He:
First, make suitable organelles that can carry out nitrification reactions.
Song He's idea is to first synthesize RNA as the raw material, and then fold the RNA to form organelles after confirming that it can produce nitration reactions. This step will take a lot of time, but the result is easy to predict. He has read a lot of relevant papers, so he should be able to do it by copying the gourd.
Second, allow the algae to accept new organelles and reproduce from generation to generation.
This step is completely unknown. The organelle all-in-one machine is only responsible for roughly injecting the organelles into the algae. As for whether the algae is dead or alive after the injection, God only knows!
Just like early breast augmentation surgeries, the silicone has been roughly padded in anyway, so I won’t be responsible for whether the breasts will become rotten in the future!
"Didi!" The instrument self-test is completed and the small green indicator light lights up.
Song He took a deep breath, tapped the instrument screen with both hands, and got busy.
As the fingers fly, the sequence of synthetic RNA strands appears.
According to the system requirements, Song He synthesized the genes of two nitrifying bacteria, namely ammonia-oxidizing bacteria and nitrite-oxidizing bacteria.
The former can convert ammonia into nitrous acid, and the latter can relay nitrous acid into nitric acid.
Both processes will allow the cells to gain some energy, but it's so little that it's a drop in the bucket or even negligible compared to algae photosynthesis.
Song He keenly realized that this step of the black technology blueprint does not require energy, but nitrogen!
In modern explosives, a common energy source for explosions is the rupture of nitrogen-nitrogen bonds. When algae undergo a nitrification reaction, they can absorb nitrogen from the environment into the body and reserve raw materials for the subsequent synthesis of explosives!
I was busy until dark.
Hungry, Song He gritted his teeth and persisted. His wrist was almost numb, and he finally created a nitrification cell organelle!
"Buddha bless! Darwin bless!"
Song He clicked the "Inject" button on the screen while praying!
A loud noise rang out from the laboratory, and the organelle all-in-one machine roared and began to inject nitrifying organelles into the algae cells that had been waiting for a long time!
The whole process takes about half an hour, just in time to have dinner.
Song He ordered takeout, 30 pot stickers.
A few minutes later, a notification popped up on the phone, indicating that the takeout was delivered.
Song He was so hungry that he almost fainted, but he still waited for two minutes before opening the laboratory door and taking off the takeout bag from the door handle.
"Damn, why don't you have chopsticks?" Song He was depressed.
He looked up at the glass rod on the test table, hesitated, rolled up his sleeves, and started eating the pot stickers.
After devouring the food, he washed his hands and returned to the instrument.
"Didi!" The small green light of the instrument lights up and the operation is completed!
Song He took out the green algae liquid and nervously observed it under the microscope.
A large number of feather diatoms are crowded together like a pile of glass test tubes filled with celery juice.
[The feathered diatom was forcibly injected with organelles and fell into weakness]
[The feathered diatom was forcibly injected with organelles and fell into weakness]
No matter how you move the angle of view, there are always signs of weakness.
These algae are like a patient who has just come off the operating table and is severely weakened.
Song He waited in fear for a long time, and what he was afraid of finally happened!
[Feather Diatom suffered a sudden death due to internal disorder! 】
[Feather Diatom suffered a sudden death due to internal disorder! 】
Massive death!
The dead feathered diatoms still maintain the shell of the glass tube, but the liquid in their bodies escapes, like gurgling green blood.
There was green everywhere in the field of vision, it was terrible!
Song He made an emergency rescue, added 10 new culture bottles, replaced them with different culture fluids, diverted the dying diatoms, and put them into a new environment for cultivation.
"Sure enough, it won't go well!" He sighed.
At the moment, we can only pray that some individual feathered diatoms survive, can resume reproduction, and form a large and vibrant group again.
Song He is not idle either. In addition to the feathered diatom he just experimented with for the first time, there are dozens of other algae to try!
He was running around alone in the laboratory, constantly extracting samples, putting them into the organelle machine, and turning it on over and over again!
…
Noon the next day.
Song He was eating takeaway steamed buns when a message came from his cell phone.
He glanced at the screen, put down his bag and stood up, ran to open the lab door and handed over the spare lab coat.
Yu Anbang walked in and took the clothes, turned around and saw the scene in the laboratory, and was so frightened that he cursed.
"Holy shit, Song He, what are you doing!"